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Addgene

pLL5.0 E-cadherin shRNA/E-cadherin GFP-IRES-HA-H-Ras V12
(Plasmid #101285)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 101285 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    LentiLox pLL5.0(backbone pLL3.7)
  • Backbone manufacturer
    J. Bear (UNC Chapel Hill, USA)
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    E-Cadherin-GFP-H-Ras
  • gRNA/shRNA sequence
    unknown
  • Species
    M. musculus (mouse)
  • Mutation
    H-Ras V12
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLL5.0 E-cadherin shRNA/E-cadherin GFP-IRES-HA-H-Ras V12 was a gift from Alpha Yap (Addgene plasmid # 101285 ; http://n2t.net/addgene:101285 ; RRID:Addgene_101285)

  • For your References section:

    Cortical F-actin stabilization generates apical-lateral patterns of junctional contractility that integrate cells into epithelia. Wu SK, Gomez GA, Michael M, Verma S, Cox HL, Lefevre JG, Parton RG, Hamilton NA, Neufeld Z, Yap AS. Nat Cell Biol. 2014 Feb;16(2):167-78. doi: 10.1038/ncb2900. Epub 2014 Jan 12. 10.1038/ncb2900 PubMed 24413434
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