pLL5.0 E-cadherin shRNA/Ecad-tdTomato
(Plasmid
#101278)
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PurposeLentiviral expression of shRNA targeting Ecad and Ecad-tdtomato
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 101278 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneLentiLox pLL5.0(backbone pLL3.7)
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Backbone manufacturerJ. Bear (UNC Chapel Hill, USA)
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameE-cadherin
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gRNA/shRNA sequenceunknown
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SpeciesM. musculus (mouse)
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Tag
/ Fusion Protein
- tdtomato (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLL5.0 E-cadherin shRNA/Ecad-tdTomato was a gift from Alpha Yap (Addgene plasmid # 101278 ; http://n2t.net/addgene:101278 ; RRID:Addgene_101278) -
For your References section:
Cortical F-actin stabilization generates apical-lateral patterns of junctional contractility that integrate cells into epithelia. Wu SK, Gomez GA, Michael M, Verma S, Cox HL, Lefevre JG, Parton RG, Hamilton NA, Neufeld Z, Yap AS. Nat Cell Biol. 2014 Feb;16(2):167-78. doi: 10.1038/ncb2900. Epub 2014 Jan 12. 10.1038/ncb2900 PubMed 24413434
