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Addgene

5VBT (UBC)
(Plasmid #101261)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 101261 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28-MHL
  • Backbone manufacturer
    SGC (Addgene Plasmid #26096)
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    UBC
  • Alt name
    5VBT
  • Alt name
    gi|132626734
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    234
  • Entrez Gene
    UBC (a.k.a. HMG20)
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis-TEV (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

N terminal tag: MHHHHHHSSGRENLYFQG. SGC Clone Sample ID: ubh04:YTC016-E10:C231906. SGC or PDB link: http://www.thesgc.org/structures/5VBT/

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    5VBT (UBC) was a gift from Cheryl Arrowsmith (Addgene plasmid # 101261 ; http://n2t.net/addgene:101261 ; RRID:Addgene_101261)
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