5UBB (LOC149281)
(Plasmid
#101257)
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PurposeBacterial expression for structure determination; may not be full ORF
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 101257 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET28-MKH8SUMO
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Backbone manufacturerSGC (Addgene Plasmid #79526)
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameMETTL11B
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Alt nameLOC149281
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Alt name5UBB
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Alt namegi|88943581
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SpeciesH. sapiens (human)
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Insert Size (bp)663
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Entrez GeneMETTL11B (a.k.a. C1orf184, HOMT1B, NTM1B)
- Promoter T7
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Tag
/ Fusion Protein
- 8xHis-Thrombin-SUMO-TEV (N terminal on backbone)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
N terminal tag: MKKHHHHHHHHSSGLVPRGSMSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLRRLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIGGTENLYFQG. SGC Clone Sample ID: LOC149281:JMC119-E02:C237152. SGC or PDB link: http://www.thesgc.org/structures/5UBB/
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
5UBB (LOC149281) was a gift from Cheryl Arrowsmith (Addgene plasmid # 101257 ; http://n2t.net/addgene:101257 ; RRID:Addgene_101257)
