pBabe-kappa-HA-dL5-myc-ADRB2
(Plasmid #101253)

• Purpose: Expresses HA-dL5(E52D)-cMyc N-terminal fusion of beta-2 adrenergic receptor in mammalian cells, MMLV retroviral plasmid (MBIC5, dL5**, FAP, ADRB2)
• Depositing Lab: Marcel Bruchez
• Publication: Perkins et al Biochemistry. 2018 Feb 6;57(5):861-871. doi: 10.1021/acs.biochem.7b01135. Epub 2018 Jan 16.

Backbone

• Vector backbone: pBABE-SacLac
• Backbone size w/o insert (bp): 6437
• Total vector size (bp): 7881
• Modifications to backbone: Sfi site removed from pBABE
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: kappa-HA-dL5-myc-ADRB2
• Species: H. sapiens (human), S. cerevisiae (budding yeast)
• Insert Size (bp): 1444
• Entrez Gene: ADRB2 (a.k.a. ADRB2R, ADRBR, ARB2, B2AR, BAR, BETA2AR)
• Promoter: MMLV LTR
• Tags / Fusion Proteins:
  • FAP-dL5 (N terminal on insert)
  • HA epitope (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: BsmI (destroyed during cloning)
• 5′ sequencing primer: BABESeqF
• 3′ sequencing primer: BABESeqR

Resource Information

• Supplemental Documents:
  • pBABE-kappa-HA-dL5-myc-ADRB2.gb
• A portion of this plasmid was derived from a plasmid made by: β2AR receptor (template: fosmid WI2-2202O9/G248P86156H5, BACPAC Resources Center, Oakland CA)
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Originally used in publication:
John Holleran, Dara Brown,* Margaret H. Fuhrman, Sally A. Adler, Gregory W. Fisher, and Jonathan W. Jarvik
Fluorogen-Activating Proteins as Biosensors of Cell-Surface Proteins in Living Cells
Cytometry A. 2010 Aug; 77(8): 776–782.