F40 tension sensor
(Plasmid #101252)

• Purpose: Expresses the F40-based tension sensor module. The biosensor allows force measurements at 1-6 pN.
• Depositing Lab: Carsten Grashoff
• Publication: Austen et al Nat Cell Biol. 2015 Dec;17(12):1597-606. doi: 10.1038/ncb3268. Epub 2015 Nov 2.

Backbone

• Vector backbone: pLPCX
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 6238
• Total vector size (bp): 7810
• Modifications to backbone: modified multicloning site
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: YPet(short)-F40-mCherry
• Alt name: F40 tension sensor module
• Insert Size (bp): 1524
• Promoter: CMV
• Tags / Fusion Proteins:
  • cysteine (N terminal on insert)
  • cysteine (C terminal on insert)
  • His-tag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: AGCTCGTTTAGTGAACCGTCAGATC
• 3′ sequencing primer: ACCTACAGGTGGGGTCTTTCATTCCC

Resource Information

• Supplemental Documents:
  • pLPCXmod + Cys-[YPet(short)-F40-mCherry]-Cys-His(tag).gb
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry