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Opto-CRAC version 1
(Plasmid #101245)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 101245 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTriEx
  • Backbone size w/o insert (bp) 5000
  • Total vector size (bp) 6570
  • Vector type
    Mammalian Expression, Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    LOV2 and STIM1 cytosolic fragment
  • Alt name
    LOVSoc
  • Species
    Synthetic
  • Insert Size (bp)
    888
  • Promoter CMV
  • Tag / Fusion Protein
    • mCherry (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer mch-for:CCC CGT AAT GCA GAA GAA GA
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Opto-CRAC version 1 was a gift from Yubin Zhou (Addgene plasmid # 101245 ; http://n2t.net/addgene:101245 ; RRID:Addgene_101245)

  • For your References section:

    Near-infrared photoactivatable control of Ca(2+) signaling and optogenetic immunomodulation. He L, Zhang Y, Ma G, Tan P, Li Z, Zang S, Wu X, Jing J, Fang S, Zhou L, Wang Y, Huang Y, Hogan PG, Han G, Zhou Y. Elife. 2015 Dec 8;4. pii: e10024. doi: 10.7554/eLife.10024. 10.7554/eLife.10024 PubMed 26646180
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