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Addgene

pHR-mCh-Cry2WT
(Plasmid #101221)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 101221 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHR
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Cry2PHR
  • Species
    A. thaliana (mustard weed)
  • Promoter SFFV
  • Tag / Fusion Protein
    • mCherry (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Our Cry2WT contains an additional 29 amino acids at the C-terminus of Cry2PHR. These additional residues are also retained in other constructs we used in the associated study.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHR-mCh-Cry2WT was a gift from Clifford Brangwynne (Addgene plasmid # 101221 ; http://n2t.net/addgene:101221 ; RRID:Addgene_101221)

  • For your References section:

    Spatiotemporal Control of Intracellular Phase Transitions Using Light-Activated optoDroplets. Shin Y, Berry J, Pannucci N, Haataja MP, Toettcher JE, Brangwynne CP. Cell. 2017 Jan 12;168(1-2):159-171.e14. doi: 10.1016/j.cell.2016.11.054. Epub 2016 Dec 29. 10.1016/j.cell.2016.11.054 PubMed 28041848
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