pJSC111 - Bacterial expression plasmid for SpCas9-HF1 variant
(Plasmid #101209)

• Purpose: Bacterial expression plasmid for SpCas9-HF1 variant
• Depositing Labs: Jennifer Doudna, Keith Joung
• Publication: Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature24268.

Backbone

• Vector backbone: pCT10
• Backbone manufacturer: Doudna lab
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SpCas9 variant N497A/R661A/Q695A/Q926A
• Alt name: Streptococcus pyogenes Cas9
• Species: Streptococcus pyogenes
• Mutation: N497A, R661A, Q695A and Q926A
• Promoter: T7
• Tags / Fusion Proteins:
  • 10x His (N terminal on backbone)
  • MBP (N terminal on backbone)
  • TEV site (N terminal on backbone)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-term

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/early/2017/08/12/160036 for bioRxiv preprint.