pDD2000
(Plasmid
#101197)
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PurposeName: pBluescript-LANApi,K14. Bidirectional reporter driving LANApi side [green luciferase isoform from pCBG68] and K14 side [red luciferase isoform from pCBR] in pBluescript backbone.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 101197 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBluescript II (KS+)
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Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 7150
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Vector typeLuciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameLANApi side [green luciferase isoform of pCBG68], K14 side [red luciferase isoform of pCBR]
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SpeciesKSHV (viral)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDD2000 was a gift from Dirk Dittmer (Addgene plasmid # 101197 ; http://n2t.net/addgene:101197 ; RRID:Addgene_101197) -
For your References section:
Quantitative analysis of the bidirectional viral G-protein-coupled receptor and lytic latency-associated nuclear antigen promoter of Kaposi's sarcoma-associated herpesvirus. Hilton IB, Dittmer DP. J Virol. 2012 Sep;86(18):9683-95. doi: 10.1128/JVI.00881-12. Epub 2012 Jun 27. 10.1128/JVI.00881-12 PubMed 22740392