pCDH-EF1-mouse SmyD1-T2A-copGFP
(Plasmid #101157)

• Purpose: Lentiviral expression of SmyD1 and copGFP
• Depositing Lab: Martine Roussel
• Publication: Wei et al Oncotarget. 2017 Jul 27. doi: 10.18632/oncotarget.19642.

Backbone

• Vector backbone: pCDH-EF1-MCS-T2A-copGFP
• Backbone manufacturer: System Biosciences
• Total vector size (bp): 8726
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SmyD1
• Alt name: Accession # BC076601clone ID30609878 (GE Dharmacon)
• Species: M. musculus (mouse)
• Entrez Gene: Smyd1 (a.k.a. 4632404M21Rik, Bop, C78565, Zmynd18)
• Promoter: EF1

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: unknown

Resource Information

• A portion of this plasmid was derived from a plasmid made by: SmyD1 synthesized by GE Dharmacon

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry