pSNAP-tag (T7) Vector
(Plasmid #101137)

• Purpose: (Empty Backbone) Expression of protein of interest with SNAP-Tag in bacterial cells
• Depositing Labs: New England Biolabs, Ana Egana
• Publication: Egana Lab NEB plasmids (unpublished)

Backbone

• Vector backbone: pSNAP-tag (T7) Vector
• Backbone size (bp): 3725
• Vector type: Bacterial Expression
• Tag / Fusion Protein:
  • SNAP-tag (SNAP26b) (N terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None
• Tag / Fusion Protein:
  • SNAP-tag (SNAP26b) (N terminal on backbone)

Resource Information

• Supplemental Documents:
  • pSNAP-tagT7.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

pSNAP-tag(T7) is a high copy number E. coli expression plasmid encoding SNAP26b, a SNAP-tag protein, which can be expressed under the control of the T7 promoter. Codon usage of the gene is optimized for expression in E. coli. The fusion partner should be cloned as a fusion to the C-terminus of the SNAP-tag. For more information on this plasmid, please see https://www.addgene.org/depositor-collections/neb-cell-imaging-tags/

This plasmid is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). For more information about commercial rights, please contact NEB's Global Business Development team at .