pCLIP-tag (m) Vector
(Plasmid #101136)

• Purpose: (Empty Backbone) Expression of protein of interest with CLIP-Tag in Mammalian cells
• Depositing Labs: New England Biolabs, Ana Egana
• Publication: Egana Lab NEB plasmids (unpublished)

Backbone

• Vector backbone: pCLIP-tag (m) Vector
• Backbone size (bp): 5805
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418) ; Kanamycin
• Tag / Fusion Protein:
  • CLIP-tag (CLIP10) (N terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None
• Tag / Fusion Protein:
  • CLIP-tag (CLIP10) (N terminal on backbone)

Resource Information

• Supplemental Documents:
  • pCLIP-tagm.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

Codon usage of the gene is optimized for expression in mammalian cells. pCLIP-tag(m) contains two multiple cloning sites to allow cloning of the fusion partner as a fusion to the N- or C-terminus of the SNAP-tag. to allow cloning of the fusion partner as a fusion to the N- or C-terminus of the SNAP-tag. For more information on this plasmid, please see https://www.addgene.org/depositor-collections/neb-cell-imaging-tags/

This plasmid is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). For more information about commercial rights, please contact NEB's Global Business Development team at .