pMCP-GPI Control Plasmid
(Plasmid #101132)

• Purpose: Expression of MCP-Tagged GPI anchor in Mammalian cells
• Depositing Labs: New England Biolabs, Ana Egana
• Publication: Egana Lab NEB plasmids (unpublished)

Backbone

• Vector backbone: pMCP
• Backbone size w/o insert (bp): 5151
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418) ; Kanamycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GPI
• Alt name: glycosylphosphatidylinositol
• Insert Size (bp): 87
• Promoter: CMV
• Tag / Fusion Protein:
  • MCP-tag (Mutant ACP- tag) (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: GACGCAAATGGGCGGTAG (CMV Forward Primer)
• 3′ sequencing primer: GCTGCGCAACTGTTGGGAA (TR1 Reverse Primer)

Resource Information

• Supplemental Documents:
  • pMCP-GPI .gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

Originally cloned by Covalys. The GPI only is 87 bp. The size of ER signal sequence + MCP+GPI is 408 bp. The MCP-tag is N-terminal to the GPI sequence. Additional resistance to Kanamycin.

For more information on this plasmid, please see https://www.addgene.org/depositor-collections/neb-cell-imaging-tags/

This plasmid is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). For more information about commercial rights, please contact NEB's Global Business Development team at .