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Addgene

pCLIPf-Cox8A Control Plasmid
(Plasmid #101130)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 101130 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *

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Backbone

  • Vector backbone
    pCLIP
  • Backbone size w/o insert (bp) 6077
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418) ; Kanamycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cox8A
  • Alt name
    Cytochrome C oxidase, subunit 8-2
  • Alt name
    COX8-2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    207
  • Entrez Gene
    COX8A (a.k.a. COX, COX8, COX8-2, COX8L, MC4DN15, VIII, VIII-L)
  • Promoter CMV
  • Tag / Fusion Protein
    • CLIP-tag (CLIPf) (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer GACGCAAATGGGCGGTAG (CMV Forward Primer)
  • 3′ sequencing primer CTGGGGCAGGCACTTCCA (SNAP/CLIP Reverse Primer)
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Originally cloned by Covalys. The CLIP-tag is an C-insert tag. Additional antibiotic resistance to Kanmycin

For more information on this plasmid, please see https://www.addgene.org/depositor-collections/neb-cell-imaging-tags/

This plasmid is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCLIPf-Cox8A Control Plasmid was a gift from New England Biolabs & Ana Egana (Addgene plasmid # 101130 ; http://n2t.net/addgene:101130 ; RRID:Addgene_101130)