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pBDKRB2_TCS(Q’G)_NLS-HA-dCas9(C)_T2A_DHFR-PCP-VP64
(Plasmid #101110)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 101110 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBDKRB2_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1
  • Modifications to backbone
    The VP64_T2A_MCP-P65-HSF1 cassette from pBDKRB2_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1 was removed, and DHFR-PCP-VP64 was placed downstream of dCas9(C) sequence separated by a T2A site. The DHFR-PCP-VP64 sequence was obtained from plasmid DHFR-PP7-VP64_T2A_GFP (gift from Amit Choudhary, Addgene plasmid #86167).
  • Vector type
    Mammalian Expression, CRISPR, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BDKRB2-dCas9(C)_DHFR-PCP-VP64
  • Tags / Fusion Proteins
    • Flag (N terminal on insert)
    • HA

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    plasmid DHFR-PP7-VP64_T2A_GFP (gift from Amit Choudhary, Addgene plasmid #86167); pBDKRB2_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBDKRB2_TCS(Q’G)_NLS-HA-dCas9(C)_T2A_DHFR-PCP-VP64 was a gift from Tudor Fulga (Addgene plasmid # 101110 ; http://n2t.net/addgene:101110 ; RRID:Addgene_101110)

  • For your References section:

    Engineering Synthetic Signaling Pathways with Programmable dCas9-Based Chimeric Receptors. Baeumler TA, Ahmed AA, Fulga TA. Cell Rep. 2017 Sep 12;20(11):2639-2653. doi: 10.1016/j.celrep.2017.08.044. 10.1016/j.celrep.2017.08.044 PubMed 28903044
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