pBDKRB2_TCS(Q’L)_HA-dCas9(N)_P2A-Puro-WPRE
(Plasmid
#101108)
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PurposeEncodes dCas9(N) fused to BDKRB2
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 101108 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepTMt_NES_TCS(Q’G)_HA-dCas9( N)_P2A-Puro-WPRE
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Modifications to backboneThe TMt, NES and TCS(Q’G) sequences from pTMt_NES_TCS(Q’G)_HA-dCas9(N)_P2A-Puro-WPRE were removed and replaced with the membrane localisation signal/FLAG tag/BDKBR2 coding sequence/V2 tail from plasmid BDKBR2-Tango and the TEV cleavage site ENLYFQL.
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Vector typeMammalian Expression, CRISPR, Synthetic Biology
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBDKRB2-dCas9(N)
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Tags
/ Fusion Proteins
- Flag (N terminal on insert)
- HA
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byBDKBR2-Tango (gift from Bryan Roth, Addgene plasmid #66230); pTMt_NES_TCS(Q’G)_HAdCas9(N)_P2A-Puro-WPRE.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBDKRB2_TCS(Q’L)_HA-dCas9(N)_P2A-Puro-WPRE was a gift from Tudor Fulga (Addgene plasmid # 101108 ; http://n2t.net/addgene:101108 ; RRID:Addgene_101108) -
For your References section:
Engineering Synthetic Signaling Pathways with Programmable dCas9-Based Chimeric Receptors. Baeumler TA, Ahmed AA, Fulga TA. Cell Rep. 2017 Sep 12;20(11):2639-2653. doi: 10.1016/j.celrep.2017.08.044. 10.1016/j.celrep.2017.08.044 PubMed 28903044