pBDKRB2_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1
(Plasmid #101107)

• Purpose: Encodes dCas9(C)-VP64 with SAM components fused to BDKRB2
• Depositing Lab: Tudor Fulga
• Publication: Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653. doi: 10.1016/j.celrep.2017.08.044.

Backbone

• Vector backbone: pTMt_TCS(Q’G)_NLSHA- dCas9(C)-VP64_T2A_MCP-P65-HSF1
• Modifications to backbone: A sequence containing the membrane localisation signal, FLAG tag, BDKBR2 coding sequence and the V2 tail were PCR amplified from plasmid BDKBR2-Tango (gift from Bryan Roth, Addgene plasmid #66230) and used to replace the TMt in pTMt_TCS(Q’G)_NLSHA- dCas9(C)-VP64_T2A_MCP-P65-HSF1.
• Vector type: Mammalian Expression, CRISPR, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: BDKRB2-dCas9(N)
• Tags / Fusion Proteins:
  • Flag (N terminal on insert)
  • HA

Resource Information

• Supplemental Documents:
  • pBDKRB2_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1.gb
• A portion of this plasmid was derived from a plasmid made by: BDKBR2-Tango (gift from Bryan Roth, Addgene plasmid #66230); pTMt_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry