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Addgene

pCMV-eBE-S3
(Plasmid #101093)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 101093 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV
  • Backbone size w/o insert (bp) 3373
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    eBE-S3
  • Alt name
    rAPOBEC1-XTEN-Cas9n-UGI-NLS-2A-UGI-NLS-2A-UGI-NLS-2A-UGI-NLS
  • Species
    R. norvegicus (rat); Bacteriophage PBS2, S. pyogenes
  • Insert Size (bp)
    6126
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer CMV
  • 3′ sequencing primer BGH poly reverse
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Derived from Addgene plasmid pCMV-BE3 (#73021)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-eBE-S3 was a gift from Jia Chen (Addgene plasmid # 101093 ; http://n2t.net/addgene:101093 ; RRID:Addgene_101093)

  • For your References section:

    Enhanced base editing by co-expression of free uracil DNA glycosylase inhibitor. Wang L, Xue W, Yan L, Li X, Wei J, Chen M, Wu J, Yang B, Yang L, Chen J. Cell Res. 2017 Aug 29. doi: 10.1038/cr.2017.111. 10.1038/cr.2017.111 PubMed 28849781
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