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PurposeExpresses UGI in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 101091 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameUGI
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Alt nameUGI-NLS
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SpeciesBacteriophage PBS2
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Insert Size (bp)276
- Promoter CMV
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Tag
/ Fusion Protein
- NLS (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer CMV
- 3′ sequencing primer BGH poly reverse (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byDerived from Addgene plasmid pCMV-BE3 (#73021)
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUGI-NLS was a gift from Jia Chen (Addgene plasmid # 101091 ; http://n2t.net/addgene:101091 ; RRID:Addgene_101091) -
For your References section:
Enhanced base editing by co-expression of free uracil DNA glycosylase inhibitor. Wang L, Xue W, Yan L, Li X, Wei J, Chen M, Wu J, Yang B, Yang L, Chen J. Cell Res. 2017 Aug 29. doi: 10.1038/cr.2017.111. 10.1038/cr.2017.111 PubMed 28849781
