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Addgene

pDR FLIP43 GPS1-NR
(Plasmid #101085)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 101085 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDR FLIP43
  • Backbone manufacturer
    PMID: 24737862
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GPS1-NR
  • Species
    A. thaliana (mustard weed), Synthetic
  • Insert Size (bp)
    2907
  • Mutation
    AtGAI E51K E54R, AtGID1C S114A F115A
  • Promoter pPMA1
  • Tags / Fusion Proteins
    • 6HIS (N terminal on insert)
    • cMyc (C terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDR FLIP43 GPS1-NR was a gift from Wolf Frommer & Alexander Jones (Addgene plasmid # 101085 ; http://n2t.net/addgene:101085 ; RRID:Addgene_101085)

  • For your References section:

    In vivo gibberellin gradients visualized in rapidly elongating tissues. Rizza A, Walia A, Lanquar V, Frommer WB, Jones AM. Nat Plants. 2017 Oct 2. doi: 10.1038/s41477-017-0021-9. 10.1038/s41477-017-0021-9 PubMed 28970478
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