Skip to main content
Addgene

p16 nlsGPS1-NR
(Plasmid #101082)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 101082 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    p16-Kan vector
  • Backbone manufacturer
    PMID: 24576426
  • Vector type
    Plant Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    nlsGPS1-NR
  • Species
    A. thaliana (mustard weed), Synthetic
  • Insert Size (bp)
    2840
  • Mutation
    AtGAI E51K E54R, AtGID1C S114A F115A
  • Promoter p16 (AT3G60245)
  • Tag / Fusion Protein
    • cMyc (C terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p16 nlsGPS1-NR was a gift from Wolf Frommer & Alexander Jones (Addgene plasmid # 101082 ; http://n2t.net/addgene:101082 ; RRID:Addgene_101082)

  • For your References section:

    In vivo gibberellin gradients visualized in rapidly elongating tissues. Rizza A, Walia A, Lanquar V, Frommer WB, Jones AM. Nat Plants. 2017 Oct 2. doi: 10.1038/s41477-017-0021-9. 10.1038/s41477-017-0021-9 PubMed 28970478
Related items:
Commonly requested with: