p16 nlsGPS1
(Plasmid
#101081)
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PurposeGA biosensor expression plasmid
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 101081 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonep16-Kan vector
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Backbone manufacturerPMID: 24576426
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Vector typePlant Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namenlsGPS1
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SpeciesA. thaliana (mustard weed), Synthetic
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Insert Size (bp)2840
- Promoter p16 (AT3G60245)
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Tag
/ Fusion Protein
- cMyc (C terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p16 nlsGPS1 was a gift from Wolf Frommer & Alexander Jones (Addgene plasmid # 101081 ; http://n2t.net/addgene:101081 ; RRID:Addgene_101081) -
For your References section:
In vivo gibberellin gradients visualized in rapidly elongating tissues. Rizza A, Walia A, Lanquar V, Frommer WB, Jones AM. Nat Plants. 2017 Oct 2. doi: 10.1038/s41477-017-0021-9. 10.1038/s41477-017-0021-9 PubMed 28970478