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pNO183
(Plasmid #101068)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 101068 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pProTet.E333
  • Total vector size (bp) 5684
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    ppsR2
  • Species
    Rhodopseudomonas palustris CGA009
  • Insert Size (bp)
    1395
  • GenBank ID
    WP_011157097.1
  • Entrez Gene
    ppsR2 (a.k.a. RPA1536)

Gene/Insert 2

  • Gene/Insert name
    bphP1
  • Alt name
    rpa1537
  • Species
    Rhodopseudomonas palustris CGA009
  • Insert Size (bp)
    2196
  • Mutation
    insert guanine at position 430 to fix frameshift deletion
  • GenBank ID

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNO183 was a gift from Jeffrey Tabor (Addgene plasmid # 101068 ; http://n2t.net/addgene:101068 ; RRID:Addgene_101068)

  • For your References section:

    Engineering an E. coli near-infrared light sensor. Ong NTX, Olson EJ, Tabor JJ. ACS Synth Biol. 2017 Nov 1. doi: 10.1021/acssynbio.7b00289. 10.1021/acssynbio.7b00289 PubMed 29091422
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