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Addgene

pcDNA-flag-hKDM4A(H188A)-polyA
(Plasmid #101052)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 101052 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Total vector size (bp) 8843
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    KDM4A
  • Alt name
    JHDM3A
  • Alt name
    JMJD2A
  • Species
    H. sapiens (human)
  • Entrez Gene
    KDM4A (a.k.a. JHDM3A, JMJD2, JMJD2A, TDRD14A)
  • Promoter T7
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA-flag-hKDM4A(H188A)-polyA was a gift from Yi Zhang (Addgene plasmid # 101052 ; http://n2t.net/addgene:101052 ; RRID:Addgene_101052)

  • For your References section:

    Histone Demethylase Expression Enhances Human Somatic Cell Nuclear Transfer Efficiency and Promotes Derivation of Pluripotent Stem Cells. Chung YG, Matoba S, Liu Y, Eum JH, Lu F, Jiang W, Lee JE, Sepilian V, Cha KY, Lee DR, Zhang Y. Cell Stem Cell. 2015 Dec 3;17(6):758-66. doi: 10.1016/j.stem.2015.10.001. Epub 2015 Oct 29. 10.1016/j.stem.2015.10.001 PubMed 26526725
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