HA-Rab8a-DN (T22N)
(Plasmid #101049)

• Purpose: Expresses HA-tagged human Rab8-DN (T22N) in mammalian cells
• Depositing Lab: Ken-Ichi Takemaru
• Publication: Burke et al J Cell Biol. 2014 Oct 13;207(1):123-37. doi: 10.1083/jcb.201406140.

Backbone

• Vector backbone: pCS2+HA
• Backbone size w/o insert (bp): 4100
• Total vector size (bp): 4730
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Rab8a
• Species: H. sapiens (human)
• Mutation: T22N
• Entrez Gene: RAB8A (a.k.a. MEL, RAB8)
• Promoter: CMV
• Tag / Fusion Protein:
  • HA (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CCCAAGCTTGATTTAG (SP6)
• 3′ sequencing primer: TTATGCTGAGTGATATC (T7)

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry