HA-Rab11-DN (S25N)
(Plasmid #101046)

• Purpose: Expresses HA-tagged human Rab11-DN (S25N) in mammalian cells
• Depositing Lab: Ken-Ichi Takemaru
• Publication: Burke et al J Cell Biol. 2014 Oct 13;207(1):123-37. doi: 10.1083/jcb.201406140.

Backbone

• Vector backbone: pCS2+HA
• Backbone size w/o insert (bp): 4100
• Total vector size (bp): 4750
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: RAB11
• Species: H. sapiens (human)
• Insert Size (bp): 650
• Mutation: S25N
• Entrez Gene: RAB11A (a.k.a. YL8)
• Promoter: CMV
• Tag / Fusion Protein:
  • HA (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CCCAAGCTTGATTTAG (SP6)
• 3′ sequencing primer: TTATGCTGAGTGATATC (T7)

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry