pEGFP-NMHC-IIA-3xA
(Plasmid #101041)

• Purpose: Expresses GFP-MYH9 construct (human myosin IIA) carrying S1943A mutation and S1916A, S1915A mutation, driven by CMV promoter. Thus inhibits CKII and PKC phosphorylation of the tail.
• Depositing Lab: Tom Egelhoff
• Publication: Rai et al J Biol Chem. 2017 Feb 24;292(8):3099-3111. doi: 10.1074/jbc.M116.733402. Epub 2017 Jan 4.

Backbone

• Vector backbone: pEGFP-C3
• Backbone manufacturer: clonetech
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Non-muscle myosin IIA heavy chain
• Alt name: MYH9
• Species: H. sapiens (human)
• Insert Size (bp): 5883
• Mutation: S1915A, S1916A, S1943A
• Entrez Gene: MYH9 (a.k.a. BDPLT6, DFNA17, EPSTS, FTNS, MATINS, MHA, NMHC-II-A, NMMHC-IIA, NMMHCA)
• Promoter: CMV
• Tag / Fusion Protein:
  • GFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (unknown if destroyed)
• 3′ cloning site: Sal (unknown if destroyed)
• 5′ sequencing primer: GGAGTTCGTGACCGCCGCCGG

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Dr. Robert Adelstein, NIHLB, NIH

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry