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Addgene

pR2Q358y
(Plasmid #101028)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 101028 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV sport
  • Backbone manufacturer
    Life technologies
  • Backbone size w/o insert (bp) 4600
  • Total vector size (bp) 8186
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Glutamate receptor subunit
  • Alt name
    Glutamate receptor 2 GluR2
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    3504
  • Mutation
    tagged with fluorescent protein
  • Entrez Gene
    Gria2 (a.k.a. GluA2, GluR-K2, GluR2, gluR-B)
  • Promoter CMV
  • Tag / Fusion Protein
    • fluorescent protein

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (unknown if destroyed)
  • 3′ cloning site Hind III (unknown if destroyed)
  • 5′ sequencing primer SP6
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pR2Q358y was a gift from Thomas Hughes (Addgene plasmid # 101028 ; http://n2t.net/addgene:101028 ; RRID:Addgene_101028)

  • For your References section:

    Regions of alpha-amino-5-methyl-3-hydroxy-4-isoxazole propionic acid receptor subunits that are permissive for the insertion of green fluorescent protein. Sheridan DL, Robert A, Cho CH, Howe JR, Hughes TE. Neuroscience. 2006 Aug 25;141(2):837-49. Epub 2006 Jun 12. 10.1016/j.neuroscience.2006.04.052 PubMed 16765522

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