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Addgene

pFlc045
(Plasmid #101017)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 101017 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    CMV sport
  • Backbone manufacturer
    life technologies
  • Backbone size w/o insert (bp) 4500
  • Total vector size (bp) 8186
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GluR 1
  • Species
    R. norvegicus (rat)
  • Mutation
    fluorescent protein inserted into subunit
  • GenBank ID
    NM_031608.1
  • Entrez Gene
    Gria1 (a.k.a. GluA1, gluR-A)
  • Promoter cmv

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ecoR1 (unknown if destroyed)
  • 3′ cloning site hind III (unknown if destroyed)
  • 5′ sequencing primer sP6
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFlc045 was a gift from Thomas Hughes (Addgene plasmid # 101017 ; http://n2t.net/addgene:101017 ; RRID:Addgene_101017)

  • For your References section:

    Regions of alpha-amino-5-methyl-3-hydroxy-4-isoxazole propionic acid receptor subunits that are permissive for the insertion of green fluorescent protein. Sheridan DL, Robert A, Cho CH, Howe JR, Hughes TE. Neuroscience. 2006 Aug 25;141(2):837-49. Epub 2006 Jun 12. 10.1016/j.neuroscience.2006.04.052 PubMed 16765522

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