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Addgene

pRH8
(Plasmid #101014)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 101014 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone size w/o insert (bp) 5400
  • Total vector size (bp) 9614
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    glutamate
  • Alt name
    gluR mutant
  • Alt name
    pRH8
  • Species
    unspecified
  • Insert Size (bp)
    2727
  • Mutation
    CHANGED THE GLUR USING PRIMERS FOUND IN ROBERT ETAL
  • Promoter CMV

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer ATGAAGATTATTTCCCCAGTTTTAA
  • 3′ sequencing primer GCCAGGTAAAGAAACCATGGCATGA
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRH8 was a gift from Thomas Hughes (Addgene plasmid # 101014 ; http://n2t.net/addgene:101014 ; RRID:Addgene_101014)

  • For your References section:

    The expression of dominant-negative subunits selectively suppresses neuronal AMPA and kainate receptors. Robert A, Hyde R, Hughes TE, Howe JR. Neuroscience. 2002;115(4):1199-210. S0306452202005341 [pii] PubMed 12453491
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