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Rp51a_SUS1 5'ss_No_BP_pBP-pUC18
(Plasmid #100990)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 100990 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC18
  • Vector type
    Bacterial Expression, Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    RP51A
  • Species
    S. cerevisiae (budding yeast)
  • Mutation
    GGTAtGT->tGTAtGa 5'SS mutant, TACTAAC->GTTAGTG BP mutataion and a TACAAAC->GTTTGTG pseudo BP mutation
  • Entrez Gene
    RPS17A (a.k.a. YML024W, RP51A, RPL51A)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer M13 forward
  • 3′ sequencing primer M13 reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Rp51a_SUS1 5'ss_No_BP_pBP-pUC18 was a gift from Aaron Hoskins (Addgene plasmid # 100990 ; http://n2t.net/addgene:100990 ; RRID:Addgene_100990)

  • For your References section:

    Dynamics and consequences of spliceosome E complex formation. Larson JD, Hoskins AA. Elife. 2017 Aug 22;6. pii: 27592. doi: 10.7554/eLife.27592. 10.7554/eLife.27592 PubMed 28829039
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