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pDRF'- AmTryoshka1;3-LS-F138I-T78H
(Plasmid #100963)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 100963 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDRf1
  • Backbone size w/o insert (bp) 6939
  • Total vector size (bp) 9869
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AmTryoshka1;3-LS-F138I-T78H
  • Alt name
    AmTryoshka-LS-FN-F138I-T78H
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    2935
  • Mutation
    F138I, T78H
  • Entrez Gene
    AMT1;3 (a.k.a. AT3G24300, AMMONIUM TRANSPORTER 1;3, ATAMT1;3, ammonium transporter 1;3)
  • Promoter PMA

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer AACAATCGTTAATAATTAATTAATTGG
  • 3′ sequencing primer GAAGTGTCAACAACGTATCTACC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDRF'- AmTryoshka1;3-LS-F138I-T78H was a gift from Wolf Frommer (Addgene plasmid # 100963 ; http://n2t.net/addgene:100963 ; RRID:Addgene_100963)

  • For your References section:

    Ratiometric Matryoshka biosensors from a nested cassette of green- and orange-emitting fluorescent proteins. Ast C, Foret J, Oltrogge LM, De Michele R, Kleist TJ, Ho CH, Frommer WB. Nat Commun. 2017 Sep 5;8(1):431. doi: 10.1038/s41467-017-00400-2. 10.1038/s41467-017-00400-2 [pii] PubMed 28874729
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