pBbE7c-XAacxnT (JBEI-14118)
(Plasmid
#100961)
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PurposeXAacx operon in pBbE7c
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 100961 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBbE7c
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH10B
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameXAacx operon
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SpeciesXanthobacter autotrophicus
- Promoter T7
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBbE7c-XAacxnT (JBEI-14118) was a gift from Jay Keasling (Addgene plasmid # 100961 ; http://n2t.net/addgene:100961 ; RRID:Addgene_100961) -
For your References section:
Engineering Bacteria to Catabolize the Carbonaceous Component of Sarin: Teaching E. coli to Eat Isopropanol. Brown ME, Mukhopadhyay A, Keasling JD. ACS Synth Biol. 2016 Dec 16;5(12):1485-1496. Epub 2016 Jul 27. 10.1021/acssynbio.6b00115 PubMed 27403844