LLP469 pEF1a-mCherry-EMPTY-gRNA
(Plasmid #100958)

• Purpose: mCherry driven by EF1a, empty gRNA scaffold driven by U6
• Depositing Lab: Ryan Lister
• Publication: Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.117. doi: 10.1101/gr.233049.117.

Backbone

• Vector backbone: LLP109 pEF1a-gRNA-GFP
• Backbone size w/o insert (bp): 3500
• Total vector size (bp): 5370
• Vector type: Mammalian Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: gRNA empty backbone with RFP
• gRNA/shRNA sequence: empty
• Species: Synthetic
• Tag / Fusion Protein:
  • RFP (N terminal on backbone)

Resource Information

• Supplemental Documents:
  • pEF1a-mCherry-gRNA.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry