LLP457 pGK-dCas9-Suntag-BFP
(Plasmid #100957)

• Purpose: BPF version of LLP339, Puro is replaced by BFP driven by EF1a, dCas9 with SungTagx10 driven by pGK, with 3xHA tag
• Depositing Lab: Ryan Lister
• Publication: Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.117. doi: 10.1101/gr.233049.117.

Backbone

• Vector backbone: LLP339 pGK-dCas9-SunTag
• Backbone size w/o insert (bp): 3570
• Total vector size (bp): 10929
• Modifications to backbone: BFP driven by pEF1a
• Vector type: Mammalian Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: dCas9, SunTag array (10xGCN4)
• Species: Synthetic; S. pyogenes
• Insert Size (bp): 7392
• Mutation: D10A, H840A for dCas9
• Tags / Fusion Proteins:
  • 3xHA (N terminal on insert)
  • BFP (C terminal on insert)

Resource Information

• Supplemental Documents:
  • pGK-dCas9-SunTag-BFP.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry