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Purpose(Empty Backbone) Cas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. KANMX marker
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 100955 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRS316
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Backbone manufacturerNA
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Vector typeYeast Expression, CRISPR, Synthetic Biology
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The protocol describing this plasmid is available at Protocol Exchange: doi:10.1038/protex.2017.021a
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJH2971 was a gift from James Haber (Addgene plasmid # 100955 ; http://n2t.net/addgene:100955 ; RRID:Addgene_100955) -
For your References section:
Cas9-mediated gene editing in Saccharomyces cerevisiae. Ranjith Anand, Gonen Memisoglu & James Haber. Protocol Exchange (2017) 10.1038/protex.2017.021a