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Purpose(Empty Backbone) Cas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. HPH marker
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 100950 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRS316
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Backbone manufacturerNA
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Vector typeYeast Expression, CRISPR, Synthetic Biology
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
bRA89 was a gift from James Haber (Addgene plasmid # 100950 ; http://n2t.net/addgene:100950 ; RRID:Addgene_100950) -
For your References section:
Rad51-mediated double-strand break repair and mismatch correction of divergent substrates. Anand R, Beach A, Li K, Haber J. Nature. 2017 Apr 20;544(7650):377-380. doi: 10.1038/nature22046. Epub 2017 Apr 12. 10.1038/nature22046 PubMed 28405019