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pETM6-RgTALsyn-HpaBC
(Plasmid #100949)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 100949 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pETM6
  • Backbone manufacturer
    Koffas Lab - RPI
  • Backbone size w/o insert (bp) 5203
  • Total vector size (bp) 9681
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    RgTALsyn
  • Alt name
    Tyrosine Ammonia Lyase
  • Alt name
    PAL
  • Alt name
    Phenylalanine Ammonia Lyase
  • Species
    Rhodotorula glutinis
  • Insert Size (bp)
    2082
  • Mutation
    Silent mutation to remove internal NdeI site on TAL
  • Promoter T7

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site SpeI (not destroyed)
  • 5′ sequencing primer GCGGCGCATATGGCGCCTCGCCCGACTTC
  • 3′ sequencing primer GCGGCGACTAGTTTATGCCAGCATCTTCAGCAGAACATTG
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    HpaB
  • Species
    E. coli
  • Insert Size (bp)
    1563
  • Promoter T7

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site SpeI (not destroyed)
  • 5′ sequencing primer ATGAAACCAGAAGATTTCCGC
  • 3′ sequencing primer TTATTTCAGCAGCTTATCCAGCATG
    • (Common Sequencing Primers)

Gene/Insert 3

  • Gene/Insert name
    HpaC
  • Species
    E. coli
  • Insert Size (bp)
    513
  • Promoter T7

Cloning Information for Gene/Insert 3

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site SpeI (not destroyed)
  • 5′ sequencing primer ATGCAATTAGATGAACAACGC
  • 3′ sequencing primer TTAAATCGCAGCTTCCATTTCC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pETM6-RgTALsyn-HpaBC was a gift from Mattheos Koffas (Addgene plasmid # 100949 ; http://n2t.net/addgene:100949 ; RRID:Addgene_100949)

  • For your References section:

    Complete Biosynthesis of Anthocyanins Using E. coli Polycultures. Jones JA, Vernacchio VR, Collins SM, Shirke AN, Xiu Y, Englaender JA, Cress BF, McCutcheon CC, Linhardt RJ, Gross RA, Koffas MAG. MBio. 2017 Jun 6;8(3). pii: e00621-17. doi: 10.1128/mBio.00621-17. 10.1128/mBio.00621-17 PubMed 28588129
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