pETM6-RgTALsyn
(Plasmid #100947)

• Purpose: Expression of Tyrosine Ammonia Lyase from inducible T7 promoter
• Depositing Lab: Mattheos Koffas
• Publication: Jones et al MBio. 2017 Jun 6;8(3). pii: e00621-17. doi: 10.1128/mBio.00621-17.

Backbone

• Vector backbone: pETM6
• Backbone manufacturer: Koffas Lab - RPI
• Backbone size w/o insert (bp): 5203
• Total vector size (bp): 7171
• Vector type: Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: RgTALsyn
• Alt name: Tyrosine Ammonia Lyase
• Alt name: PAL
• Alt name: Phenylalanine Ammonia Lyase
• Species: Rhodotorula glutinis
• Insert Size (bp): 2082
• Mutation: Silent mutation to remove internal NdeI site on TAL
• Promoter: T7

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: SpeI (not destroyed)
• 5′ sequencing primer: GCGGCGCATATGGCGCCTCGCCCGACTTC
• 3′ sequencing primer: GCGGCGACTAGTTTATGCCAGCATCTTCAGCAGAACATTG

Resource Information

• Supplemental Documents:
  • pETM6-RgTALsyn.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry