-
PurposedCas9 vector with SunTagx10, driven by pGK, with 3xHA tag, Puro driven by EF1a
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 100943 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backboneLLP249 pGK-dCas9-DNMT3a V3
- Backbone size w/o insert (bp) 5675
- Total vector size (bp) 10815
-
Modifications to backbonePuro driven by pEF1a
-
Vector typeMammalian Expression, CRISPR
-
Selectable markersPuromycin
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert namedCas9, SunTag array (10xGCN4)
-
SpeciesSynthetic; S. pyogenes
-
Insert Size (bp)1596
-
MutationD10A, H840A for dCas9
-
Tag
/ Fusion Protein
- 3xHA (N terminal on insert)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
LLP339 pGK-dCas9-SunTag was a gift from Ryan Lister (Addgene plasmid # 100943 ; http://n2t.net/addgene:100943 ; RRID:Addgene_100943) -
For your References section:
A modular dCas9-SunTag DNMT3A epigenome editing system overcomes pervasive off-target activity of direct fusion dCas9-DNMT3A constructs. Pflueger C, Tan D, Swain T, Nguyen TV, Pflueger J, Nefzger C, Polo JM, Ford E, Lister R. Genome Res. 2018 Jun 15. pii: gr.233049.117. doi: 10.1101/gr.233049.117. 10.1101/gr.233049.117 PubMed 29907613