LLP252 pEF1a-NLS-scFvGCN4-DNMT3a
(Plasmid #100941)

• Purpose: Single chain variable fragment antibody GCN4 fused to DNMT3a, binds SunTag, with 3xTy1 tag
• Depositing Lab: Ryan Lister
• Publication: Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.117. doi: 10.1101/gr.233049.117.

Backbone

• Vector backbone: LLP245 pEF1a-TALE-DNMT3a-Wt
• Backbone size w/o insert (bp): 4321
• Total vector size (bp): 7190
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: scFvGCN4, sfGFP, GB1, DNMT3a catalytic domain
• Species: H. sapiens (human), Synthetic
• Insert Size (bp): 5157
• Mutation: D10A, H840A for dCas9
• Entrez Gene: DNMT3A (a.k.a. DNMT3A2, HESJAS, M.HsaIIIA, TBRS)
• Tags / Fusion Proteins:
  • 3xTy1 (C terminal on insert)
  • 1xHA (N terminal on insert)

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

scFvGCN4 domain obtained from pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS (Addgene Plasmid #60904)