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PurposeSingle chain variable fragment antibody GCN4 fused to DNMT3a, binds SunTag, with 3xTy1 tag
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Depositing Lab
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Sequence Information
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Sequences (2) — Accept Affinity Reagent Sequence Policy
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Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 100941 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneLLP245 pEF1a-TALE-DNMT3a-Wt
- Backbone size w/o insert (bp) 4321
- Total vector size (bp) 7190
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namescFvGCN4, sfGFP, GB1, DNMT3a catalytic domain
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SpeciesH. sapiens (human), Synthetic
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Insert Size (bp)5157
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MutationD10A, H840A for dCas9
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Entrez GeneDNMT3A (a.k.a. DNMT3A2, HESJAS, M.HsaIIIA, TBRS)
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Tags
/ Fusion Proteins
- 3xTy1 (C terminal on insert)
- 1xHA (N terminal on insert)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
scFvGCN4 domain obtained from pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS (Addgene Plasmid #60904)
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
LLP252 pEF1a-NLS-scFvGCN4-DNMT3a was a gift from Ryan Lister (Addgene plasmid # 100941 ; http://n2t.net/addgene:100941 ; RRID:Addgene_100941) -
For your References section:
A modular dCas9-SunTag DNMT3A epigenome editing system overcomes pervasive off-target activity of direct fusion dCas9-DNMT3A constructs. Pflueger C, Tan D, Swain T, Nguyen TV, Pflueger J, Nefzger C, Polo JM, Ford E, Lister R. Genome Res. 2018 Jun 15. pii: gr.233049.117. doi: 10.1101/gr.233049.117. 10.1101/gr.233049.117 PubMed 29907613
Map uploaded by the depositor.
