LLP249 pGK-dCas9-DNMT3a V3
(Plasmid #100938)

• Purpose: dCas9 and DNMT WT driven by pGK promoter and Puromycin driven by EF1a
• Depositing Lab: Ryan Lister
• Publication: Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.117. doi: 10.1101/gr.233049.117.

Backbone

• Vector backbone: LLP185 pLVP-dCas9-DNMT3a V2
• Backbone size w/o insert (bp): 5654
• Total vector size (bp): 11127
• Vector type: Mammalian Expression, Lentiviral, CRISPR
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: dCas9, DNMT3a catalytic domain
• Species: H. sapiens (human); S.pyogenes
• Insert Size (bp): 5157
• Mutation: D10A, H840A for dCas9
• Entrez Gene: DNMT3A (a.k.a. DNMT3A2, HESJAS, M.HsaIIIA, TBRS)
• Tags / Fusion Proteins:
  • 3xHA (N terminal on insert)
  • 3xTy1 (C terminal on insert)

Resource Information

• Supplemental Documents:
  • pGK-dCas9-DNMT3A wt -V3.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This V3 construct has Puro selection expressed via pEF1a promoter