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LLP245 pEF1a-TALE-DNMT3a-Wt
(Plasmid #100937)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 100937 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Unkown
  • Backbone manufacturer
    Ethan Ford
  • Backbone size w/o insert (bp) 4295
  • Total vector size (bp) 6239
  • Vector type
    Mammalian Expression, TALEN

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TALE insertion site, DNMT3a catalytic domain
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    1923
  • Entrez Gene
    DNMT3A (a.k.a. DNMT3A2, HESJAS, M.HsaIIIA, TBRS)
  • Tags / Fusion Proteins
    • 3xHA (N terminal on insert)
    • 3xTy1 (C terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LLP245 pEF1a-TALE-DNMT3a-Wt was a gift from Ryan Lister (Addgene plasmid # 100937 ; http://n2t.net/addgene:100937 ; RRID:Addgene_100937)

  • For your References section:

    A modular dCas9-SunTag DNMT3A epigenome editing system overcomes pervasive off-target activity of direct fusion dCas9-DNMT3A constructs. Pflueger C, Tan D, Swain T, Nguyen TV, Pflueger J, Nefzger C, Polo JM, Ford E, Lister R. Genome Res. 2018 Jun 15. pii: gr.233049.117. doi: 10.1101/gr.233049.117. 10.1101/gr.233049.117 PubMed 29907613
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