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Addgene

LLP109 pEF1a-gRNA-GFP
(Plasmid #100935)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 100935 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    gRNA_Cloning Vector
  • Backbone manufacturer
    Addgene Plasmid #41824
  • Total vector size (bp) 6036
  • Vector type
    Mammalian Expression ; gRNA scaffold
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    gRNA scaffold
  • Alt name
    gRNA
  • Species
    Synthetic
  • Insert Size (bp)
    362

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LLP109 pEF1a-gRNA-GFP was a gift from Ryan Lister (Addgene plasmid # 100935 ; http://n2t.net/addgene:100935 ; RRID:Addgene_100935)

  • For your References section:

    A modular dCas9-SunTag DNMT3A epigenome editing system overcomes pervasive off-target activity of direct fusion dCas9-DNMT3A constructs. Pflueger C, Tan D, Swain T, Nguyen TV, Pflueger J, Nefzger C, Polo JM, Ford E, Lister R. Genome Res. 2018 Jun 15. pii: gr.233049.117. doi: 10.1101/gr.233049.117. 10.1101/gr.233049.117 PubMed 29907613
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