pcDNA3-Q769gp120-8his
(Plasmid #100929)

• Purpose: Mammalian expression plasmid for gp120 subunit from the Q769.d22 HIV-1 strain
• Depositing Lab: Erik Procko
• Publication: Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.

Backbone

• Vector backbone: pcDNA3.1(+)
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5344
• Total vector size (bp): 6889
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HIV-1 (Q769.d22) Env
• Alt name: gp120
• Alt name: Env
• Species: Human immunodeficiency virus 1
• Insert Size (bp): 1545
• Mutation: Codon-optimized synthetic gene
• GenBank ID: AAM66234.1
• Promoter: CMV
• Tags / Fusion Proteins:
  • CD5 leader sequence (N terminal on insert)
  • Purification tag after K510 is AGGHHHHHHHH (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV_fwd
• 3′ sequencing primer: BGH-rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry