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Addgene

relAE335Q_NS1
(Plasmid #100917)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 100917 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pam1303
  • Backbone size w/o insert (bp) 5700
  • Total vector size (bp) 7400
  • Vector type
    Cyanobacteria cloning vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin and Streptomycin, 50 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    RelA fragment from E.coli with point mutation E335Q
  • Species
    E. coli
  • Insert Size (bp)
    1740
  • Mutation
    E 335 converted to Q
  • Tag / Fusion Protein
    • FLAG (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    relAE335Q_NS1 was a gift from Erin O'Shea (Addgene plasmid # 100917 ; http://n2t.net/addgene:100917 ; RRID:Addgene_100917)

  • For your References section:

    ppGpp Controls Global Gene Expression in Light and in Darkness in S. elongatus. Puszynska AM, O'Shea EK. Cell Rep. 2017 Dec 12;21(11):3155-3165. doi: 10.1016/j.celrep.2017.11.067. 10.1016/j.celrep.2017.11.067 PubMed 29241543
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