spoT_NS1
(Plasmid
#100914)
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Purposeexpresses spoT (from native promoter) from the NS1 site in the S. elongatus PCC7942 genome
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 100914 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepAM1303
- Backbone size w/o insert (bp) 5700
- Total vector size (bp) 9000
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Vector typeCyanobacteria cloning vector
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin and Streptomycin, 50 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namesynpcc7942_1377 (spoT)
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SpeciesS. elongatus PCC7942
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Insert Size (bp)3140
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer -
- 3′ sequencing primer - (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
spoT_NS1 was a gift from Erin O'Shea (Addgene plasmid # 100914 ; http://n2t.net/addgene:100914 ; RRID:Addgene_100914) -
For your References section:
ppGpp Controls Global Gene Expression in Light and in Darkness in S. elongatus. Puszynska AM, O'Shea EK. Cell Rep. 2017 Dec 12;21(11):3155-3165. doi: 10.1016/j.celrep.2017.11.067. 10.1016/j.celrep.2017.11.067 PubMed 29241543
