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Addgene

spoT-
(Plasmid #100913)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 100913 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBR322
  • Backbone size w/o insert (bp) 4361
  • Total vector size (bp) 7400
  • Vector type
    Cloning vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Kanamycin casette (in place of spoT gene) flanked by S. elongatus sequence
  • Species
    S. elongatus PCC7942
  • Insert Size (bp)
    1022

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    spoT- was a gift from Erin O'Shea (Addgene plasmid # 100913 ; http://n2t.net/addgene:100913 ; RRID:Addgene_100913)

  • For your References section:

    ppGpp Controls Global Gene Expression in Light and in Darkness in S. elongatus. Puszynska AM, O'Shea EK. Cell Rep. 2017 Dec 12;21(11):3155-3165. doi: 10.1016/j.celrep.2017.11.067. 10.1016/j.celrep.2017.11.067 PubMed 29241543
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