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Addgene

pET28a(+)/FbGH17B
(Plasmid #100912)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 100912 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a(+)
  • Backbone manufacturer
    EMD Millipore
  • Backbone size w/o insert (bp) 5369
  • Total vector size (bp) 6167
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    FbGH17Bcat
  • Species
    Formosa B
  • Insert Size (bp)
    798
  • Mutation
    not full length
  • GenBank ID
    AOR29491.1
  • Tag / Fusion Protein
    • His-Tag (N terminal on backbone)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer CAGCGGCCTGGTGCCGCGCGGCAGCCATACTTTAAATAAGGGTCTGCAC
  • 3′ sequencing primer CTCAGTGGTGGTGGTGGTGGTGCTCGAGTTATTGAAATTTTAAATTCTCATTC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28a(+)/FbGH17B was a gift from Jan-Hendrik Hehemann (Addgene plasmid # 100912 ; http://n2t.net/addgene:100912 ; RRID:Addgene_100912)
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